Trends in Gel Electrophoresis
نویسنده
چکیده
U.V. scanning may readily be performed on unstained agar-gel strips in the dry state. Polyacrylamide gels tend to become brittle if dried; whether they can be reliably scanned in the undried state, as unstained strips or columns, depends on whether there are troubles due to deformation of the pattern when the gel is manipulated, or to optical opacity. With proteins, if not with RNA, u.v.-sensitivity in relation to polyacrylamide ‘background noise’ may be hopeless. Dye-binding methods are therefore customary, despite interpretative complications due to differences among macromolecules in dyebinding capacity. At least it seems best to avoid the additional interpretative uncertainties that are inherent in the use of columns as distinct from slabs.
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